Morphogens enable interacting supracellular phases that generate organ architecture

• 成果类型: Article
• 署名作者: Yang, Sichen; Palmquist, Karl H.; Nathan, Levy; Pfeifer, Charlotte R.; Schultheiss, Paula J.; Sharma, Anurag; Kam, Lance C.; Miller, Pearson W.; Shyer, Amy E.; Rodrigues, Alan R.
• 署名单位: Rockefeller University; Columbia University; Columbia University; Rockefeller University; Simons Foundation; Flatiron Institute
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-10020
• DOI: 10.1126/science.adg5579
• 发表日期: 2023-11-24
• 关键词: embryonic-tissues; cell; bmps; gradient; pattern; models; GROWTH; energy; roles

摘要

During vertebrate organogenesis, increases in morphological complexity are tightly coupled to morphogen expression. In this work, we studied how morphogens influence self-organizing processes at the collective or supra-cellular scale in avian skin. We made physical measurements across length scales, which revealed morphogen-enabled material property differences that were amplified at supracellular scales in comparison to cellular scales. At the supracellular scale, we found that fibroblast growth factor (FGF) promoted solidification of tissues, whereas bone morphogenetic protein (BMP) promoted fluidity and enhanced mechanical activity. Together, these effects created basement membrane-less compartments within mesenchymal tissue that were mechanically primed to drive avian skin tissue budding. Understanding this multiscale process requires the ability to distinguish between proximal effects of morphogens that occur at the cellular scale and their functional effects, which emerge at the supracellular scale.