Patterns of recombination in snakes reveal a tug-of-war between PRDM9 and promoter-like features

• 成果类型: Article
• 署名作者: Hoge, Carla; de Manuel, Marc; Mahgoub, Mohamed; Okami, Naima; Fuller, Zachary; Banerjee, Shreya; Baker, Zachary; Mcnulty, Morgan; Andolfatto, Peter; Macfarlan, Todd S.; Schumer, Molly; Tzika, Athanasia C.; Przeworski, Molly
• 署名单位: Columbia University; National Institutes of Health (NIH) - USA; NIH Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD); Stanford University; Columbia University; Howard Hughes Medical Institute; University of Geneva; Washington University (WUSTL); University of California System; University of California Davis; 23andMe, Inc.; University of Cambridge
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-11650
• DOI: 10.1126/science.adj7026
• 发表日期: 2024-02-23
• 关键词: multiple sequence alignment; meiotic recombination; phylogenetic analysis; genome annotation; cpg islands; EVOLUTION; initiation; landscape; hotspots; scale

摘要

In some mammals, notably humans, recombination occurs almost exclusively where the protein PRDM9 binds, whereas in vertebrates lacking an intact PRDM9, such as birds and canids, recombination rates are elevated near promoter-like features. To determine whether PRDM9 directs recombination in nonmammalian vertebrates, we focused on an exemplar species with a single, intact PRDM9 ortholog, the corn snake (Pantherophis guttatus). Analyzing historical recombination rates along the genome and crossovers in pedigrees, we found evidence that PRDM9 specifies the location of recombination events, but we also detected a separable effect of promoter-like features. These findings reveal that the uses of PRDM9 and promoter-like features need not be mutually exclusive and instead reflect a tug-of-war that is more even in some species than others.