Recording physiological history of cells with chemical labeling

• 成果类型: Article
• 署名作者: Huppertz, Magnus-Carsten; Wilhelm, Jonas; Grenier, Vincent; Schneider, Martin W.; Falt, Tjalda; Porzberg, Nicola; Hausmann, David; Hoffmann, Dirk C.; Hai, Ling; Tarnawski, Miroslaw; Pino, Gabriela; Slanchev, Krasimir; Kolb, Ilya; Acuna, Claudio; Fenk, Lisa M.; Baier, Herwig; Hiblot, Julien; Johnsson, Kai
• 署名单位: Max Planck Society; Helmholtz Association; German Cancer Research Center (DKFZ); Ruprecht Karls University Heidelberg; Helmholtz Association; German Cancer Research Center (DKFZ); Ruprecht Karls University Heidelberg; National Center for Tumor Diseases; Ruprecht Karls University Heidelberg; Helmholtz Association; German Cancer Research Center (DKFZ); Max Planck Society; Ruprecht Karls University Heidelberg; Swiss Federal Institutes of Technology Domain; Ecole Polytechnique Federale de Lausanne
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-11451
• DOI: 10.1126/science.adg0812
• 发表日期: 2024-02-23
• 页码: 890-897
• 关键词: gene-expression; in-vivo; reveals; calcium; protein; transgenesis; refinement; circuits; program; tango

摘要

Recordings of the physiological history of cells provide insights into biological processes, yet obtaining such recordings is a challenge. To address this, we introduce a method to record transient cellular events for later analysis. We designed proteins that become labeled in the presence of both a specific cellular activity and a fluorescent substrate. The recording period is set by the presence of the substrate, whereas the cellular activity controls the degree of the labeling. The use of distinguishable substrates enabled the recording of successive periods of activity. We recorded protein-protein interactions, G protein-coupled receptor activation, and increases in intracellular calcium. Recordings of elevated calcium levels allowed selections of cells from heterogeneous populations for transcriptomic analysis and tracking of neuronal activities in flies and zebrafish.