Sister chromatid cohesion is mediated by individual cohesin complexes

• 成果类型: Article
• 署名作者: Ochs, Fena; Green, Charlotte; Szczurek, Aleksander Tomasz; Pytowski, Lior; Kolesnikova, Sofia; Brown, Jill; Gerlich, Daniel Wolfram; Buckle, Veronica; Schermelleh, Lothar; Nasmyth, Kim Ashley
• 署名单位: University of Oxford; University of Oxford; Austrian Academy of Sciences; Vienna Biocenter (VBC); Institute of Molecular Biotechnology (IMBA); Medical University of Vienna; Vienna Biocenter (VBC); University of Vienna; Medical University of Vienna; University of Oxford; University of Copenhagen
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-10797
• DOI: 10.1126/science.adl4606
• 发表日期: 2024-03-08
• 页码: 1122-1130
• 关键词: dna; sororin; replication; chromosomes; sequence; proteins; anaphase; genome; tool

摘要

Eukaryotic genomes are organized by loop extrusion and sister chromatid cohesion, both mediated by the multimeric cohesin protein complex. Understanding how cohesin holds sister DNAs together, and how loss of cohesion causes age-related infertility in females, requires knowledge as to cohesin's stoichiometry in vivo. Using quantitative super-resolution imaging, we identified two discrete populations of chromatin-bound cohesin in postreplicative human cells. Whereas most complexes appear dimeric, cohesin that localized to sites of sister chromatid cohesion and associated with sororin was exclusively monomeric. The monomeric stoichiometry of sororin:cohesin complexes demonstrates that sister chromatid cohesion is conferred by individual cohesin rings, a key prediction of the proposal that cohesion arises from the co-entrapment of sister DNAs.