Periodic cytokinin responses in Lotus japonicus rhizobium infection and nodule development

• 成果类型: Article
• 署名作者: Soyano, Takashi; Akamatsu, Akira; Takeda, Naoya; Watahiki, Masaaki K.; Goh, Tatsuaki; Okuma, Nao; Suganuma, Norio; Kojima, Mikiko; Takebayashi, Yumiko; Sakakibara, Hitoshi; Nakajima, Keiji; Kawaguchi, Masayoshi
• 署名单位: National Institutes of Natural Sciences (NINS) - Japan; National Institute for Basic Biology (NIBB); Graduate University for Advanced Studies - Japan; Kwansei Gakuin University; Hokkaido University; Nara Institute of Science & Technology; Aichi University Education; RIKEN; Nagoya University; RIKEN
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-12852
• DOI: 10.1126/science.adk5589
• 发表日期: 2024-07-19
• 页码: 288-294
• 关键词: transcription factor; gene-expression; negative regulator; insensitive mutant; binary vectors; gras proteins; root; nodulation; legume; organogenesis

摘要

Host plants benefit from legume root nodule symbiosis with nitrogen-fixing bacteria under nitrogen-limiting conditions. In this interaction, the hosts must regulate nodule numbers and distribution patterns to control the degree of symbiosis and maintain root growth functions. The host response to symbiotic bacteria occurs discontinuously but repeatedly at the region behind the tip of the growing roots. Here, live-imaging and transcriptome analyses revealed oscillating host gene expression with approximately 6-hour intervals upon bacterial inoculation. Cytokinin response also exhibited a similar oscillation pattern. Cytokinin signaling is crucial to maintaining the periodicity, as observed in cytokinin receptor mutants displaying altered infection foci distribution. This periodic regulation influences the size of the root region responsive to bacteria, as well as the nodulation process progression.