PI4P-mediated solid-like Merlin condensates orchestrate Hippo pathway regulation

• 成果类型: Article
• 署名作者: Guo, Pengfei; Li, Bing; Dong, Wei; Zhou, Huabin; Wang, Li; Su, Ting; Carl, Christopher; Zheng, Yonggang; Hong, Yang; Deng, Hua; Pan, Duojia
• 署名单位: Howard Hughes Medical Institute; University of Texas System; University of Texas Southwestern Medical Center; Pennsylvania Commonwealth System of Higher Education (PCSHE); University of Pittsburgh; University of Texas System; University of Texas Southwestern Medical Center; Howard Hughes Medical Institute
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-12850
• DOI: 10.1126/science.adf4478
• 发表日期: 2024-08-09
• 关键词: tumor-suppressor protein; planar cell polarity; plasma-membrane; drosophila homologs; neurofibromatosis 2; tissue homeostasis; structural basis; yap oncoprotein; domain; ORGANIZATION

摘要

Despite recent studies implicating liquid-like biomolecular condensates in diverse cellular processes, many biomolecular condensates exist in a solid-like state, and their function and regulation are less understood. We show that the tumor suppressor Merlin, an upstream regulator of the Hippo pathway, localizes to both cell junctions and medial apical cortex in Drosophila epithelia, with the latter forming solid-like condensates that activate Hippo signaling. Merlin condensation required phosphatidylinositol-4-phosphate (PI4P)-mediated plasma membrane targeting and was antagonistically controlled by Pez and cytoskeletal tension through plasma membrane PI4P regulation. The solid-like material properties of Merlin condensates are essential for physiological function and protect the condensates against external perturbations. Collectively, these findings uncover an essential role for solid-like condensates in normal physiology and reveal regulatory mechanisms for their formation and disassembly.