Hebbian instruction of axonal connectivity by endogenous correlated spontaneous activity

• 成果类型: Article
• 署名作者: Matsumoto, Naoyuki; Barson, Daniel; Liang, Liang; Crair, Michael C.
• 署名单位: Yale University; Yale University; Yale University; Yale University
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-10386
• DOI: 10.1126/science.adh7814
• 发表日期: 2024-08-16
• 关键词: in-vivo; patterned activity; retinal waves; branch dynamics; mice lacking; plasticity; receptor; cortex; synaptogenesis; architecture

摘要

Spontaneous activity refines neural connectivity prior to the onset of sensory experience, but it remains unclear how such activity instructs axonal connectivity with subcellular precision. We simultaneously measured spontaneous retinal waves and the activity of individual retinocollicular axons and tracked morphological changes in axonal arbors across hours in vivo in neonatal mice. We demonstrate that the correlation of an axon branch's activity with neighboring axons or postsynaptic neurons predicts whether the branch will be added, stabilized, or eliminated. Desynchronizing individual axons from their local networks, changing the pattern of correlated activity, or blocking N-methyl-d-aspartate receptors all significantly altered single-axon morphology. These observations provide the first direct evidence in vivo that endogenous patterns of correlated neuronal activity instruct fine-scale refinement of axonal processes.