Identification of cambium stem cell factors and their positioning mechanism

• 成果类型: Article
• 署名作者: Eswaran, Gugan; Zhang, Xixi; Rutten, Jacob Pieter; Han, Jingyi; Iida, Hiroyuki; Ortiz, Jennifer Lopez; Makila, Riikka; Wybouw, Brecht; Jimenez, Benjamin Planterose; Vainio, Leo; Porcher, Alexis; Gavarron, Marina Leal; Zhang, Jing; Blomster, Tiina; Wang, Xin; Dolan, David; Smetana, Ondrej; Brady, Siobhan M.; Topcu, Melis Kucukoglu; ten Tusscher, Kirsten; Etchells, J. Peter; Mahonen, Ari Pekka
• 署名单位: University of Helsinki; University of Helsinki; University of Helsinki; Utrecht University; Durham University; University of California System; University of California Davis; Utrecht University; University of Bergen
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-9148
• DOI: 10.1126/science.adj8752
• 发表日期: 2024-11-08
• 页码: 646-653
• 关键词: arabidopsis root; secondary growth; wood formation; cle peptides; gene; diffusion; aintegumenta; proteins; wall; pxy

摘要

Wood constitutes the largest reservoir of terrestrial biomass. Composed of xylem, it arises from one side of the vascular cambium, a bifacial stem cell niche that also produces phloem on the opposing side. It is currently unknown which molecular factors endow cambium stem cell identity. Here we show that TRACHEARY ELEMENT DIFFERENTIATION INHIBITORY FACTOR (TDIF) ligand-activated PHLOEM INTERCALATED WITH XYLEM (PXY) receptors promote the expression of CAMBIUM-EXPRESSED AINTEGUMENTA-LIKE (CAIL) transcription factors to define cambium stem cell identity in the Arabidopsis root. By sequestrating the phloem-originated TDIF, xylem-expressed PXY confines the TDIF signaling front, resulting in the activation of CAIL expression and stem cell identity in only a narrow domain. Our findings show how signals emanating from cells on opposing sides ensure robust yet dynamically adjustable positioning of a bifacial stem cell layer.