Chemically induced proximity reveals a Piezo-dependent meiotic checkpoint at the oocyte nuclear envelope

• 成果类型: Article
• 署名作者: Liu, Chenshu; Dernburg, Abby F.
• 署名单位: University of California System; University of California Berkeley; University of California System; University of California Berkeley; United States Department of Energy (DOE); Lawrence Berkeley National Laboratory; Lehigh University
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-13993
• DOI: 10.1126/science.adm7969
• 发表日期: 2024-11-22
• 关键词: programmed cell-death; c-elegans; phosphorylation sites; chromosome synapsis; telomere attachment; meiosis; protein; translocation; DYNAMICS; genome

摘要

Sexual reproduction relies on robust quality control during meiosis. Assembly of the synaptonemal complex between homologous chromosomes (synapsis) regulates meiotic recombination and is crucial for accurate chromosome segregation in most eukaryotes. Synapsis defects can trigger cell cycle delays and, in some cases, apoptosis. We developed and deployed a chemically induced proximity system to identify key elements of this quality control pathway in Caenorhabditis elegans. Persistence of the polo-like kinase PLK-2 at pairing centers-specialized chromosome regions that interact with the nuclear envelope-induced apoptosis of oocytes in response to phosphorylation and destabilization of the nuclear lamina. Unexpectedly, the Piezo1/PEZO-1 channel localized to the nuclear envelope and was required to transduce this signal to promote apoptosis in maturing oocytes.