The complete telomere-to-telomere sequence of a mouse genome

• 成果类型: Article
• 署名作者: Liu, Junli; Li, Qilin; Hu, Yixuan; Yu, Yi; Zheng, Kai; Li, Dengfeng; Qin, Lexin; Yu, Xiaochun
• 署名单位: Westlake Laboratory; Westlake University; Westlake University
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-11781
• DOI: 10.1126/science.adq8191
• 发表日期: 2024-12-06
• 页码: 1141-1146
• 关键词: embryonic stem-cells; satellite dna; minor satellite; centromere; ORGANIZATION; generation; draft; mice

摘要

The current reference genome of Mus musculus, GRCm39, has major gaps in both euchromatic and heterochromatic regions associated with repetitive sequences. In this work, we have sequenced and assembled the telomere-to-telomere genome of mouse haploid embryonic stem cells. The results reveal more than 7.7% of previously uncovered sequences of the mouse genome, including ribosomal DNA arrays and pericentromeric and subtelomeric regions, as well as an additional 140 genes predicted to be protein-coding. This study helps to address knowledge gaps in the mouse genome.