Active-reset protein sensors enable continuous in vivo monitoring of inflammation

• 成果类型: Article
• 署名作者: Zargartalebi, H.; Mirzaie, S.; Ghavaminejad, A.; Ahmed, S. U.; Esmaeili, F.; Geraili, A.; Flynn, C. D.; Chang, D.; Das, J.; Abdrabou, A.; Sargent, E. H.; Kelley, S. O.
• 署名单位: Northwestern University; Northwestern University; University of Toronto; Northwestern University; Robert H. Lurie Comprehensive Cancer Center; University of Toronto; Chan Zuckerberg Initiative (CZI); Northwestern University
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-10739
• DOI: 10.1126/science.adn2600
• 发表日期: 2024-12-06
• 页码: 1146-1153
• 关键词: dna aptamer

摘要

Continuous measurement of proteins in vivo is important for real-time disease management and prevention. Implantable sensors for monitoring small molecules such as glucose have been available for more than a decade. However, analysis of proteins remains an unmet need because the lower physiological levels require that sensors have high affinities, which are linked to long complexation half-lives (t(1/2) similar to 20 hours) and slow equilibration when concentrations decrease. We report active-reset sensors by use of high-frequency oscillations to accelerate dissociation, which enables regeneration of the unbound form of the sensor within 1 minute. When implemented within implanted devices, these sensors allow for real-time, in vivo monitoring of proteins within interstitial fluid. Active-reset protein sensors track biomarker levels on a physiological timescale for inflammation monitoring in living animals.