A canonical protein complex controls immune homeostasis and multipathogen resistance

• 成果类型: Article
• 署名作者: Wu, Yue; Xu, Weiying; Zhao, Guoyan; Lei, Ziyao; Li, Kui; Liu, Jiyun; Huang, Shijia; Wang, Junli; Zhong, Xiangbin; Yin, Xin; Wang, Yuandong; Zhang, Haochen; He, Yang; Ye, Zian; Meng, Yonggang; Chang, Xiaoyu; Lin, Hui; Wang, Xin; Gao, Yuanyuan; Chai, Jijie; Parker, Jane E.; Deng, Yiwen; Zhang, Yu; Gao, Mingjun; He, Zuhua
• 署名单位: Chinese Academy of Sciences; Center for Excellence in Molecular Plant Sciences, CAS; Chinese Academy of Sciences; University of Chinese Academy of Sciences, CAS; ShanghaiTech University; Fudan University; Westlake University; Max Planck Society; Zhengzhou University; Henan Normal University; Zhejiang University
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-9550
• DOI: 10.1126/science.adr2138
• 发表日期: 2024-12-20
• 页码: 1405-1412
• 关键词: disease resistance; tir domains; receptors; RECOGNITION

摘要

The calcium (Ca2+) sensor ROD1 (RESISTANCE OF RICE TO DISEASES1) is a master regulator of immunity in rice. By screening suppressors of rod1 mutants, we show that ROD1 governs immune homeostasis by surveilling the activation of a canonical immune pathway. Mutations in OsTIR (TIR-only protein), OsEDS1 (enhanced disease susceptibility 1), OsPAD4 (phytoalexin deficient 4), and OsADR1 (activated disease resistance 1) all abolish enhanced disease resistance of rod1 plants. OsTIR catalyzes the production of second messengers 2 '-(5 ''-phosphoribosyl)-5 '-adenosine monophosphate (pRib-AMP) and diphosphate (pRib-ADP), which trigger formation of an OsEDS1-OsPAD4-OsADR1 (EPA) immune complex. ROD1 interacts with OsTIR and inhibits its enzymatic activity, whereas mutation of ROD1 leads to constitutive activation of the EPA complex. Thus, we unveil an immune network that fine-tunes immune homeostasis and multipathogen resistance in rice.