Evolution of interorganismal strigolactone biosynthesis in seed plants

• 成果类型: Article
• 署名作者: Zhou, Anqi; Kane, Annalise; Wu, Sheng; Wang, Kaibiao; Santiago, Michell; Ishiguro, Yui; Yoneyama, Kaori; Palayam, Malathy; Shabek, Nitzan; Xie, Xiaonan; Nelson, David C.; Li, Yanran
• 署名单位: University of California System; University of California San Diego; University of California System; University of California Riverside; Shanghai University of Traditional Chinese Medicine; Utsunomiya University; Saitama University; University of California System; University of California Davis
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-12410
• DOI: 10.1126/science.adp0779
• 发表日期: 2025-01-17
• 页码: 263-+
• 关键词: escherichia-coli; arabidopsis; proteins; receptor; germination; carlactone; vectors; dwarf14; max1; tool

摘要

Strigolactones (SLs) are methylbutenolide molecules derived from beta-carotene through an intermediate carlactonoic acid (CLA). Canonical SLs act as signals to microbes and plants, whereas noncanonical SLs are primarily plant hormones. The cytochrome P450 CYP722C catalyzes a critical step, converting CLA to canonical SLs in most angiosperms. Using synthetic biology, we investigated the function of CYP722A, an evolutionary predecessor of CYP722C. CYP722A converts CLA into 16-hydroxy-CLA (16-OH-CLA), a noncanonical SL detected exclusively in the shoots of various flowering plants. 16-OH-CLA application restores control of shoot branching to SL-deficient mutants in Arabidopsis thaliana and is perceived by the SL signaling pathway. We hypothesize that biosynthesis of 16-OH-CLA by CYP722A was a metabolic stepping stone in the evolution of canonical SLs that mediate rhizospheric signaling in many flowering plants.