Ectoderm barcoding reveals neural and cochlear compartmentalization

• 成果类型: Article
• 署名作者: de Haan, Sandra; He, Jingyan; Corbat, Agustin A.; Belicova, Lenka; Ratz, Michael; Vinsland, Elin; Frisen, Jonas; Kelley, Matthew W.; Andersson, Emma R.
• 署名单位: Karolinska Institutet; National Institutes of Health (NIH) - USA; NIH National Institute on Deafness & Other Communication Disorders (NIDCD); Leiden University; Leiden University Medical Center (LUMC); Leiden University - Excl LUMC; Karolinska Institutet
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-13577
• DOI: 10.1126/science.adq9248
• 发表日期: 2025-04-04
• 页码: 60-68
• 关键词: supporting cells; inner-ear; regeneration; hearing

摘要

Placodes and the neural crest are defining features of vertebrates. In this study, we investigate their lineages in mice using in utero approaches. We demonstrated that nanoinjection at embryonic day 7.5 targeted the ectoderm, including the future nervous system, placodes, and neural crest, allowing highly efficient manipulation of the future nervous system and inner ear. By using heritable DNA barcodes and high-throughput next-generation single-cell lineage tracing, we elucidated convergent differentiation pathways and identified distinct nervous system-, neural crest-, and otic placode-derived lineages. Clonal analyses identified early neural and cochlear compartmentalization, linking differentiated cell types to their progenitors or cellular siblings. This provides foundational insights for neuroscience and developmental biology.