Cryo-EM reveals mechanisms of natural RNA multivalency

• 成果类型: Article
• 署名作者: Wang, Liu; Xie, Jiahao; Gong, Tao; Wu, Hao; Tu, Yifan; Peng, Xin; Shang, Sitong; Jia, Xinyu; Ma, Haiyun; Zou, Jian; Xu, Sheng; Zheng, Xin; Zhang, Dong; Liu, Yang; Zhang, Chong; Luo, Yongbo; Huang, Zirui; Shao, Bin; Ying, Binwu; Cheng, Yu; Guo, Yingqiang; Lai, Ying; Huang, Dingming; Liu, Jianquan; Wei, Yuquan; Sun, Siqi; Zhou, Xuedong; Su, Zhaoming
• 署名单位: Sichuan University; Sichuan University; Sichuan University; Fudan University; Sichuan University; Sichuan University; Chinese University of Hong Kong; Sichuan University
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-10901
• DOI: 10.1126/science.adv3451
• 发表日期: 2025-05-01
• 页码: 545-550
• 关键词: refinement

摘要

Homo-oligomerization of biological macromolecules leads to functional assemblies that are critical to understanding various cellular processes. However, RNA quaternary structures have rarely been reported. Comparative genomics analysis has identified RNA families containing hundreds of sequences that adopt conserved secondary structures and likely fold into complex three-dimensional structures. In this study, we used cryo-electron microscopy (cryo-EM) to determine structures from four RNA families, including ARRPOF and OLE forming dimers and ROOL and GOLLD forming hexameric, octameric, and dodecameric nanostructures, at 2.6- to 4.6-angstrom resolutions. These homo-oligomeric assemblies reveal a plethora of structural motifs that contribute to RNA multivalency, including kissing-loop, palindromic base-pairing, A-stacking, metal ion coordination, pseudoknot, and minor-groove interactions. These results provide the molecular basis of intermolecular interactions driving RNA multivalency with potential functional relevance.