Interphase cell morphology defines the mode, symmetry, and outcome of mitosis

• 成果类型: Article
• 署名作者: Lovegrove, Holly E.; Hulmes, Georgia E.; Ghadaouia, Sabrina; Revell, Christopher; Giralt-Pujol, Marta; Alhashem, Zain; Pena, Andreia; Nogare, Damian D.; Appleton, Ellen; Costa, Guilherme; Mort, Richard L.; Ballestrem, Christoph; Jones, Gareth W.; Manning, Cerys S.; Chitnis, Ajay B.; Franco, Claudio A.; Linker, Claudia; Bentley, Katie; Herbert, Shane P.
• 署名单位: University of Manchester; Yale University; Francis Crick Institute; University of Manchester; Boston University; Boston University; University of Leeds; University of London; King's College London; Universidade de Lisboa; National Institutes of Health (NIH) - USA; NIH Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD); Human Technopole; Queens University Belfast; Lancaster University; Universidade Catolica Portuguesa; University of London; King's College London; University of Leeds
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-10332
• DOI: 10.1126/science.adu9628
• 发表日期: 2025-05-01
• 关键词: dna segregation; division; shape; geometry; BEHAVIOR

摘要

During tissue formation, dynamic cell shape changes drive morphogenesis while asymmetric divisions create cellular diversity. We found that the shifts in cell morphology that shape tissues could concomitantly act as conserved instructive cues that trigger asymmetric division and direct core identity decisions underpinning tissue building. We performed single-cell morphometric analyses of endothelial and other mesenchymal-like cells. Distinct morphological changes switched cells to an isomorphic mode of division, which preserved pre-mitotic morphology throughout mitosis. In isomorphic divisions, interphase morphology appeared to provide a geometric code defining mitotic symmetry, fate determinant partitioning, and daughter state. Rab4-positive endosomes recognized this code, allowing them to respond to pre-mitotic morphology and segregate determinants accordingly. Thus, morphogenetic shape change sculpts tissue form while also generating cellular heterogeneity, thereby driving tissue assembly.