CTC1-STN1-TEN1 controls DNA break repair pathway choice via DNA end resection blockade

• 成果类型: Article
• 署名作者: Rogers, Cody M.; Kaur, Hardeep; Swift, Michelle L.; Raina, Vivek B.; Zhou, Shuo; Kawale, Ajinkya S.; Syed, Shahrez; Kelly, Korilynn G.; Jasper, Angela M.; Salunkhe, Sameer; Kwon, Youngho; Wang, Jeffrey; Shabestari, Aida Badamchi; Daley, James M.; Sacks, Adam; Gaczynska, Maria E.; Osmulski, Pawel A.; Rawal, Yashpal; Tomimatsu, Nozomi; Gayther, Simon A.; Lawrenson, Kate; Burma, Sandeep; Wasmuth, Elizabeth V.; Olsen, Shaun K.; Zhao, Weixing; Hromas, Robert; Libich, David S.; Mazin, Alexander V.; Zhou, Daohong; Greene, Eric C.; Chowdhury, Dipanjan; Sung, Patrick
• 署名单位: University of Texas System; University of Texas at San Antonio; University of Texas System; University of Texas at San Antonio; Harvard University; Harvard Medical School; Harvard University Medical Affiliates; Dana-Farber Cancer Institute; NewYork-Presbyterian Hospital; Columbia University; University of Texas System; University of Texas at San Antonio; University of Texas System; University of Texas at San Antonio; University of Texas System; University of Texas at San Antonio; University of Texas System; University of Texas at San Antonio; Harvard University; Harvard University Medical Affiliates; Massachusetts General Hospital; University of Michigan System; University of Michigan
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-11757
• DOI: 10.1126/science.adt3034
• 发表日期: 2025-05-22
• 页码: 881-888
• 关键词: double-strand breaks; homologous recombination; 53bp1; brca1; mechanism; reveals; complex

摘要

Antagonistic activities of the 53BP1 axis and the tumor suppressor BRCA1-BARD1 determine whether DNA double-strand breaks (DSBs) are repaired by end joining or homologous recombination. We show that the CTC1-STN1-TEN1 (CST) complex, a central 53BP1 axis component, suppresses DNA end resection by EXO1 and the BLM-DNA2 helicase-nuclease complex but acts by distinct mechanisms in restricting these entities. Whereas BRCA1-BARD1 alleviates the CST-imposed EXO1 blockade, it has little effect on BLM-DNA2 restriction. CST mutants impaired for DNA binding or BLM-EXO1 interaction exhibit a hyper-resection phenotype and render BRCA1-deficient cells resistant to poly(ADP-ribose) polymerase (PARP) inhibitors. Our findings mechanistically define the crucial role of CST in DNA DSB repair pathway choice and have implications for understanding cancer therapy resistance stemming from dysfunction of the 53BP1 axis.