Identification of proliferating neural progenitors in the adult human hippocampus

• 成果类型: Article
• 署名作者: Dumitru, Ionut; Paterlini, Marta; Zamboni, Margherita; Ziegenhain, Christoph; Giatrellis, Sarantis; Saghaleyni, Rasool; Bjoerklund, Asa; Alkass, Kanar; Tata, Mathew; Druid, Henrik; Sandberg, Rickard; Frisen, Jonas
• 署名单位: Karolinska Institutet; Chalmers University of Technology; Karolinska Institutet; Karolinska Institutet; Cancer Research UK
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-13960
• DOI: 10.1126/science.adu9575
• 发表日期: 2025-07-03
• 页码: 58-63
• 关键词: rna-seq; neurogenesis; platform; CHILDREN; neurons

摘要

Continuous adult hippocampal neurogenesis is involved in memory formation and mood regulation but is challenging to study in humans. Difficulties finding proliferating progenitor cells called into question whether and how new neurons may be generated. We analyzed the human hippocampus from birth through adulthood by single-nucleus RNA sequencing. We identified all neural progenitor cell stages in early childhood. In adults, using antibodies against the proliferation marker Ki67 and machine learning algorithms, we found proliferating neural progenitor cells. Furthermore, transcriptomic data showed that neural progenitors were localized within the dentate gyrus. The results contribute to understanding neurogenesis in adult humans.