Stereo-cell: Spatial enhanced-resolution single-cell sequencing with high-density DNA nanoball-patterned arrays

• 成果类型: Article
• 署名作者: Liao, Sha; Zhou, Xiaoxi; Liu, Chuanyu; Liu, Chang; Hao, Shijie; Luo, Hongyu; Hou, Huan; Liu, Qian; Zhang, Zhe; Xiao, Liyun; Xu, Yuan; Huang, Yaling; Zhou, Sining; Li, Xuerong; Wang, Yang; Xie, Lulin; Zhou, Zhichun; Dong, Shichen; Wang, Yiru; Xu, Xiaojing; Guo, Pengcheng; Lin, Xiumei; Lei, Jiajie; Wang, Qiaoling; Gong, Yuxin; Cheng, Jiaming; Yuan, Zixin; Yang, Yongqing; Huang, Zhi; Li, Shenglong; Zheng, Yuhui; Yang, Shichen; Huang, Xin; Liu, Weiqing; Li, Mei; Deng, Zhonghan; Yang, Xinyu; Yin, Jianhua; Luo, Yingjie; Lai, Yiwei; Yuan, Yue; Cheng, Mengnan; Wang, Bo; Ji, Jiansong; Esteban, Miguel A.; Li, Yuxiang; Gu, Ying; Ruan, Yijun; Chen, Liang; Wang, Xiangdong; Xie, Jun; Wang, Jian; Liu, Longqi; Chen, Ao; Xu, Xun
• 署名单位: Beijing Genomics Institute (BGI); Beijing Genomics Institute (BGI); Beijing Genomics Institute (BGI); Shanxi Medical University; Fujian Medical University; South China University of Technology; Chinese University of Hong Kong; Beijing Genomics Institute (BGI); Chinese Academy of Sciences; University of Chinese Academy of Sciences, CAS; South Central Minzu University; Nankai University; Huazhong Agricultural University; Beijing Genomics Institute (BGI); Beijing Genomics Institute (BGI); Wenzhou Medical University; Beijing Genomics Institute (BGI); Zhejiang University; Fudan University; Shanxi Medical University; Beijing Genomics Institute (BGI)
• 刊物名称: SCIENCE
• ISSN/ISSBN: 0036-12770
• DOI: 10.1126/science.adr0475
• 发表日期: 2025-08-21
• 关键词: growth-differentiation factor-9; t-cells; rna-seq; neuromuscular-junction; transcriptomic atlas; skeletal-muscle; diffusion maps; expression; gene; complex

摘要

Single-cell sequencing technologies have advanced our understanding of cellular heterogeneity and biological complexity. However, existing methods face limitations in throughput, capture uniformity, cell size flexibility, and technical extensibility. We present Stereo-cell, a spatial enhanced-resolution single-cell sequencing platform based on high-density DNA nanoball (DNB)-patterned arrays, which enables scalable and unbiased cell capture at a wide input range and supports high-fidelity transcriptome profiling. Stereo-cell further allows integration with imaging-based modalities and multiomics strategies, including immunofluorescence and epitope profiling. This platform is also compatible with profiling extracellular vesicles, microstructures, and large cells, whereas its spatial resolution facilitates in situ analysis of cell-cell interactions, cellular microenvironments, and subcellular transcript localization. Together, Stereo-cell provides a flexible framework for expanding single-cell research applications.