Multimodal cell atlas of the ageing human skeletal muscle

• 成果类型: Article
• 署名作者: Lai, Yiwei; Ramirez-Pardo, Ignacio; Isern, Joan; An, Juan; Perdiguero, Eusebio; Serrano, Antonio L.; Li, Jinxiu; Garcia-Dominguez, Esther; Segales, Jessica; Guo, Pengcheng; Lukesova, Vera; Andres, Eva; Zuo, Jing; Yuan, Yue; Liu, Chuanyu; Vina, Jose; Domenech-Fernandez, Julio; Gomez-Cabrera, Mari Carmen; Song, Yancheng; Liu, Longqi; Xu, Xun; Munoz-Canoves, Pura; Esteban, Miguel A.
• 署名单位: Beijing Genomics Institute (BGI); Pompeu Fabra University; Chinese Academy of Sciences; Guangzhou Institute of Biomedicine & Health, CAS; Chinese Academy of Sciences; University of Science & Technology of China, CAS; Chinese Academy of Sciences; University of Chinese Academy of Sciences, CAS; University of Valencia; CIBER - Centro de Investigacion Biomedica en Red; CIBERFES; Jilin University; University of Navarra; Guangdong Pharmaceutical University; ICREA; Guangzhou Medical University
• 刊物名称: Nature
• ISSN/ISSBN: 0028-3834
• DOI: 10.1038/s41586-024-07348-6
• 发表日期: 2024-05-02
• 页码: 154-+
• 关键词: denervation; expression; features; binding; predict; index

摘要

Muscle atrophy and functional decline (sarcopenia) are common manifestations of frailty and are critical contributors to morbidity and mortality in older people(1). Deciphering the molecular mechanisms underlying sarcopenia has major implications for understanding human ageing(2). Yet, progress has been slow, partly due to the difficulties of characterizing skeletal muscle niche heterogeneity (whereby myofibres are the most abundant) and obtaining well-haracterized human samples(3,4). Here we generate a single-cell/single-nucleus transcriptomic and chromatin accessibility map of human limb skeletal muscles encompassing over 387,000 cells/nuclei from individuals aged 15 to 99 years with distinct fitness and frailty levels. We describe how cell populations change during ageing, including the emergence of new populations in older people, and the cell-specific and multicellular network features (at the transcriptomic and epigenetic levels) associated with these changes. On the basis of cross-comparison with genetic data, we also identify key elements of chromatin architecture that mark susceptibility to sarcopenia. Our study provides a basis for identifying targets in the skeletal muscle that are amenable to medical, pharmacological and lifestyle interventions in late life.