Brainstem Dbh+ neurons control allergen-induced airway hyperreactivity

• 成果类型: Article
• 署名作者: Su, Yujuan; Xu, Jinhao; Zhu, Ziai; Chin, Jisun; Xu, Le; Yu, Haoze; Nudell, Victoria; Dash, Barsha; Moya, Esteban A.; Ye, Li; Nimmerjahn, Axel; Sun, Xin
• 署名单位: University of California System; University of California San Diego; University of California System; University of California San Diego; Scripps Research Institute; La Jolla Institute for Immunology; University of California System; University of California San Diego; Salk Institute
• 刊物名称: Nature
• ISSN/ISSBN: 0028-4595
• DOI: 10.1038/s41586-024-07608-5
• 发表日期: 2024-07-18
• 关键词: nucleus-tractus-solitarius; sensory neurons; identification; ventilation; saporin

摘要

Exaggerated airway constriction triggered by repeated exposure to allergen, also called hyperreactivity, is a hallmark of asthma. Whereas vagal sensory neurons are known to function in allergen-induced hyperreactivity(1-3), the identity of downstream nodes remains poorly understood. Here we mapped a full allergen circuit from the lung to the brainstem and back to the lung. Repeated exposure of mice to inhaled allergen activated the nuclei of solitary tract (nTS) neurons in a mast cell-, interleukin-4 (IL-4)- and vagal nerve-dependent manner. Single-nucleus RNA sequencing, followed by RNAscope assay at baseline and allergen challenges, showed that a Dbh(+) nTS population is preferentially activated. Ablation or chemogenetic inactivation of Dbh(+) nTS neurons blunted hyperreactivity whereas chemogenetic activation promoted it. Viral tracing indicated that Dbh(+) nTS neurons project to the nucleus ambiguus (NA) and that NA neurons are necessary and sufficient to relay allergen signals to postganglionic neurons that directly drive airway constriction. Delivery of noradrenaline antagonists to the NA blunted hyperreactivity, suggesting noradrenaline as the transmitter between Dbh(+) nTS and NA. Together, these findings provide molecular, anatomical and functional definitions of key nodes of a canonical allergen response circuit. This knowledge informs how neural modulation could be used to control allergen-induced airway hyperreactivity.