Methyl-reducing methanogenesis by a thermophilic culture of Korarchaeia
成果类型：
Article
署名作者：
Krukenberg, Viola; Kohtz, Anthony J.; Jay, Zackary J.; Hatzenpichler, Roland
署名单位：
Montana State University System; Montana State University Bozeman; Montana State University System; Montana State University Bozeman; Montana State University System; Montana State University Bozeman
刊物名称：
Nature
ISSN/ISSBN：
0028-6462
DOI：
10.1038/s41586-024-07829-8
发表日期：
2024-08-29
关键词：
in-situ hybridization
multiple sequence alignment
oligonucleotide probes
h-2 production
methane
identification
performance
EVOLUTION
archaea
QUALITY
摘要：
Methanogenesis mediated by archaea is the main source of methane, a strong greenhouse gas, and thus is critical for understanding Earth's climate dynamics. Recently, genes encoding diverse methanogenesis pathways have been discovered in metagenome-assembled genomes affiliated with several archaeal phyla1-7. However, all experimental studies on methanogens are at present restricted to cultured representatives of the Euryarchaeota. Here we show methanogenic growth by a member of the lineage Korarchaeia within the phylum Thermoproteota (TACK superphylum)5-7. Following enrichment cultivation of 'Candidatus Methanodesulfokora washburnenis' strain LCB3, we used measurements of metabolic activity and isotope tracer conversion to demonstrate methanol reduction to methane using hydrogen as an electron donor. Analysis of the archaeon's circular genome and transcriptome revealed unique modifications in the energy conservation pathways linked to methanogenesis, including enzyme complexes involved in hydrogen and sulfur metabolism. The cultivation and characterization of this new group of archaea is critical for a deeper evaluation of the diversity, physiology and biochemistry of methanogens. We show methanogenic growth by a member of the lineage Korarchaeia and, following cultivation, methanol reduction to methane using hydrogen as an electron donor, which could be critical for a thorough evaluation of methanogens.