Crypt density and recruited enhancers underlie intestinal tumour initiation

• 成果类型: Article
• 署名作者: Gaynor, Liam; Singh, Harshabad; Tie, Guodong; Badarinath, Krithika; Madha, Shariq; Mancini, Andrew; Bhattacharya, Swarnabh; Hoshino, Mikio; de Sauvage, Frederic J.; Murata, Kazutaka; Jadhav, Unmesh; Shivdasani, Ramesh A.
• 署名单位: Harvard University; Harvard University Medical Affiliates; Dana-Farber Cancer Institute; Harvard University; Harvard University Medical Affiliates; Dana-Farber Cancer Institute; Harvard University; Harvard Medical School; Harvard University; Harvard Medical School; Roche Holding; Genentech; Roche Holding USA; National Center for Neurology & Psychiatry - Japan; Harvard University
• 刊物名称: Nature
• ISSN/ISSBN: 0028-2485
• DOI: 10.1038/s41586-024-08573-9
• 发表日期: 2025-04-03
• 关键词: stem-cells; seq data; apc; colon; differentiation; homeostasis; adenomas; Mutation; platform; polyclonality

摘要

Oncogenic mutations that drive colorectal cancer can be present in healthy intestines for long periods without overt consequence1,2. Mutation of Apc, the most common initiating event in conventional adenomas3, activates Wnt signalling, thus conferring fitness on mutant intestinal stem cells (ISCs)4,5. Apc mutations may occur in ISCs that arise by routine self-renewal or by dedifferentiation of their progeny. Although ISCs of these different origins are fundamentally similar6,7, it is unclear whether both generate tumours equally well in uninjured intestines. It is also unknown whether cis-regulatory elements are substantively modulated upon Wnt hyperactivation or as a feature of subsequent tumours. Here we show in two mouse models that adenomas are not an obligatory outcome of Apc deletion in either ISC source, but require proximity of mutant intestinal crypts. Reduced crypt density abrogates, and aggregation of mutant colonic crypts augments, adenoma formation. Moreover, adenoma-resident ISCs open chromatin at thousands of enhancers that are inaccessible in Apc-null ISCs that are not associated with adenomas. These cis elements explain adenoma-selective gene activity and persist, with little further expansion of the repertoire, as other oncogenic mutations accumulate. Thus, cooperativity between neighbouring mutant crypts and new accessibility at specific enhancers are key steps early in intestinal tumorigenesis.